On-demand, competing gradient arrays for neutrophil chemotaxis.

ABSTRACT: Neutrophils are the most abundant type of white blood cells in the circulation, protecting the body against pathogens and responding early to inflammation. Although we understand how neutrophils respond to individual stimuli, we know less about how they prioritize between competing signals or respond to combinational signals. This situation is due in part to the lack of adequate experimental systems to provide signals in controlled spatial and temporal fashion. To address these limitations, we designed a platform for generating on-demand, competing chemical gradients and for monitoring neutrophil migration. On this platform, we implemented forty-eight assays generating independent gradients and employed synchronized valves to control the timing of these gradients. We observed faster activa

SUBMITTER: Cho H 

PROVIDER: S-EPMC3950309 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature