DJ-1 upregulates breast cancer cell invasion by repressing KLF17 expression.

ABSTRACT:

Background

DJ-1 (PARK7) was reported as an oncogene in a Ras-dependent manner. Recent studies have shown that DJ-1 stimulates cell proliferation, cell invasion, and cancer metastasis. However, the molecular mehchanism by which DJ-1 induces cancer cell invasion and metastasis remains unclear.

Methods

Breast cancer cells were transfected with DJ-1 siRNA or DJ-1 overexpression to investigate the effect of DJ-1 on KLF17 expression. ID-1 luciferase promoter assay was performed to evaluate DJ-1-dependent KLF17 expression changes. In addition, Epistasis analysis of DJ-1 and KLF17 was performed to evaluate their regulatory interactions. Ras inhibitors were pretreated to determine whether DJ-1 regulates cell invasion in a Ras-dependent manner.

Results

I n the present study, we

SUBMITTER: Ismail IA 

PROVIDER: S-EPMC3950878 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature