Polymer-enhanced delivery increases adenoviral gene expression in an orthotopic model of bladder cancer.

ABSTRACT: Gene therapy has garnered significant attention as a therapeutic approach for bladder cancer but efficient delivery and gene expression remain major hurdles. The goal of this study was to determine if cationic polymers can enhance adenoviral gene expression in cells that are difficult to transduce in vitro and to subsequently investigate lead candidates for their capacity to increase adenoviral gene expression in an orthotopic in vivo model of bladder cancer. In vitro screening of linear polyamine-based and aminoglycoside-based polymer libraries identified several candidates that enhanced adenoviral reporter gene expression in vitro. The polyamine-based polymer NPGDE-1,4 Bis significantly enhanced adenoviral gene expression in the orthotopic model of bladder cancer but unfortunately furthe

SUBMITTER: Gosnell H 

PROVIDER: S-EPMC3952388 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature