Novel lipid droplet-associated serine hydrolase regulates macrophage cholesterol mobilization.

ABSTRACT:

Objective

Lipid-laden macrophages or foam cells are characterized by massive cytosolic lipid droplet (LD) deposition containing mostly cholesterol ester (CE) derived from the lipoproteins cleared from the arterial wall. Cholesterol efflux from foam cells is considered to be atheroprotective. Because cholesterol is effluxed as free cholesterol, CE accumulation in LDs may limit free cholesterol efflux. Our objective was to identify proteins that regulate cholesterol trafficking through LDs.

Approach and results

In a proteomic analysis of the LD fraction of RAW 264.7 macrophages, we identified an evolutionarily conserved protein with a canonical GXSXG lipase catalytic motif and a predicted α/β-hydrolase fold, the RIKEN cDNA 1110057K04 gene, which we named LD-associated hydrolas

SUBMITTER: Goo YH 

PROVIDER: S-EPMC3952429 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature