Xenodiagnosis to detect Borrelia burgdorferi infection: a first-in-human study.

ABSTRACT:

Background

Animal studies suggest that Borrelia burgdorferi, the agent of Lyme disease, may persist after antibiotic therapy and can be detected by various means including xenodiagnosis using the natural tick vector (Ixodes scapularis). No convincing evidence exists for the persistence of viable spirochetes after recommended courses of antibiotic therapy in humans. We determined the safety of using I. scapularis larvae for the xenodiagnosis of B. burgdorferi infection in humans.

Methods

Laboratory-reared larval I. scapularis ticks were placed on 36 subjects and allowed to feed to repletion. Ticks were tested for B. burgdorferi by polymerase chain reaction (PCR), culture, and/or isothermal amplification followed by PCR and electrospray ionization mass spectroscopy. In additio

SUBMITTER: Marques A 

PROVIDER: S-EPMC3952603 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature