DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells.

ABSTRACT: Chromosome breakage is a major threat to genome integrity. The most accurate way to repair DNA double strand breaks (DSB) is homologous recombination (HR) with an intact copy of the broken locus. Mobility of the broken DNA has been seen to increase during the search for a donor copy. Observing chromosome dynamics during the earlier steps of HR, mainly the resection from DSB ends that generates recombinogenic single strands, requires a visualization system that does not interfere with the process, and is small relative to the few kilobases of DNA that undergo processing. Current visualization tools, based on binding of fluorescent repressor proteins to arrays of specific binding sites, have the major drawback that highly-repeated DNA and lengthy stretches of strongly bound protein can obstr

SUBMITTER: Saad H 

PROVIDER: S-EPMC3952824 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature