NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa.

ABSTRACT: The functional relationship between the formation of hundreds of fusion pores during the acrosome reaction in spermatozoa and the mobilization of calcium from the acrosome has been determined only partially. Hence, the second messenger NAADP, promoting efflux of calcium from lysosome-like compartments and one of its potential molecular targets, the two-pore channel 1 (TPC1), were analyzed for its involvement in triggering the acrosome reaction using a TPCN1 gene-deficient mouse strain. The present study documents that TPC1 and NAADP-binding sites showed a colocalization at the acrosomal region and that treatment of spermatozoa with NAADP resulted in a loss of the acrosomal vesicle that showed typical properties described for TPCs: Registered responses were not detectable for its chemical a

SUBMITTER: Arndt L 

PROVIDER: S-EPMC3952862 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature