Influence of in vitro and in vivo oxygen modulation on β cell differentiation from human embryonic stem cells.

ABSTRACT: The possibility of using human embryonic stem (hES) cell-derived β cells as an alternative to cadaveric islets for the treatment of type 1 diabetes is now widely acknowledged. However, current differentiation methods consistently fail to generate meaningful numbers of mature, functional β cells. In order to address this issue, we set out to explore the role of oxygen modulation in the maturation of pancreatic progenitor (PP) cells differentiated from hES cells. We have previously determined that oxygenation is a powerful driver of murine PP differentiation along the endocrine lineage of the pancreas. We hypothesized that targeting physiological oxygen partial pressure (pO2) levels seen in mature islets would help the differentiation of PP cells along the β-cell lineage. This hypothesis was

SUBMITTER: Cechin S 

PROVIDER: S-EPMC3952934 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature