Genetic characterization of hepatitis C virus in long-term RNA replication using Li23 cell culture systems.

ABSTRACT:

Background

The most distinguishing genetic feature of hepatitis C virus (HCV) is its remarkable diversity and variation. To understand this feature, we previously performed genetic analysis of HCV in the long-term culture of human hepatoma HuH-7-derived HCV RNA-replicating cell lines. On the other hand, we newly established HCV RNA-replicating cell lines using human hepatoma Li23 cells, which were distinct from HuH-7 cells.

Methodology/principal findings

Li23-derived HCV RNA-replicating cells were cultured for 4 years. We performed genetic analysis of HCVs recovered from these cells at 0, 2, and 4 years in culture. Most analysis was performed in two separate parts: one part covered from the 5'-terminus to NS2, which is mostly nonessential for RNA replication, and the other p

SUBMITTER: Kato N 

PROVIDER: S-EPMC3953375 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature