Localization of Cacna1s to ON bipolar dendritic tips requires mGluR6-related cascade elements.

ABSTRACT:

Purpose

L-type voltage gated calcium channels in retina localize primarily at the presynaptic active zones of photoreceptors and bipolar cells where they modulate glutamate release. However, the pore forming subunit Cacna1s of certain L-type channels is also expressed postsynaptically at the tips of ON bipolar cell dendrites where it colocalizes with mGluR6, but has an unknown function. At these dendritic tips, the components of the mGluR6 signaling cascade cluster together in a macromolecular complex, and each one's localization often depends on that of the others. Thus, we explored if Cacna1s is part of the mGluR6 complex.

Methods

We determined Cacna1s expression by PCR using an ON bipolar library, by Western blotting, and by standard immunohistochemistry.

Results

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SUBMITTER: Tummala SR 

PROVIDER: S-EPMC3954003 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature