Quantitation of glucocorticoid receptor DNA-binding dynamics by single-molecule microscopy and FRAP.

ABSTRACT: Recent advances in live cell imaging have provided a wealth of data on the dynamics of transcription factors. However, a consistent quantitative description of these dynamics, explaining how transcription factors find their target sequences in the vast amount of DNA inside the nucleus, is still lacking. In the present study, we have combined two quantitative imaging methods, single-molecule microscopy and fluorescence recovery after photobleaching, to determine the mobility pattern of the glucocorticoid receptor (GR) and the mineralocorticoid receptor (MR), two ligand-activated transcription factors. For dexamethasone-activated GR, both techniques showed that approximately half of the population is freely diffusing, while the remaining population is bound to DNA. Of this DNA-bound populati

SUBMITTER: Groeneweg FL 

PROVIDER: S-EPMC3954550 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature