Analyzing clonal variation of monoclonal antibody-producing CHO cell lines using an in silico metabolomic platform.

ABSTRACT: Monoclonal antibody producing Chinese hamster ovary (CHO) cells have been shown to undergo metabolic changes when engineered to produce high titers of recombinant proteins. In this work, we have studied the distinct metabolism of CHO cell clones harboring an efficient inducible expression system, based on the cumate gene switch, and displaying different expression levels, high and low productivities, compared to that of the parental cells from which they were derived. A kinetic model for CHO cell metabolism was further developed to include metabolic regulation. Model calibration was performed using intracellular and extracellular metabolite profiles obtained from shake flask batch cultures. Model simulations of intracellular fluxes and ratios known as biomarkers revealed significant change

SUBMITTER: Ghorbaniaghdam A 

PROVIDER: S-EPMC3954614 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature