Chromatin connectivity maps reveal dynamic promoter-enhancer long-range associations.

ABSTRACT: In multicellular organisms, transcription regulation is one of the central mechanisms modelling lineage differentiation and cell-fate determination. Transcription requires dynamic chromatin configurations between promoters and their corresponding distal regulatory elements. It is believed that their communication occurs within large discrete foci of aggregated RNA polymerases termed transcription factories in three-dimensional nuclear space. However, the dynamic nature of chromatin connectivity has not been characterized at the genome-wide level. Here, through a chromatin interaction analysis with paired-end tagging approach using an antibody that primarily recognizes the pre-initiation complexes of RNA polymerase II, we explore the transcriptional interactomes of three mouse cells of prog

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3954713 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature