Adhesion molecule expression and function of primary endothelial cells in benign and malignant tissues correlates with proliferation.

ABSTRACT:

Background

Comparative analysis of the cellular biology of the microvasculature in different tissues requires the availability of viable primary endothelial cells (ECs). This study describes a novel method to isolate primary ECs from healthy organs, repair blastemas and tumors as examples of non-proliferating and proliferating benign and malignant tissues and their functional characterization.

Methodology/principal findings

Single cell suspensions from hearts, lungs, repair blastemas and tumors were incubated consecutively with an anti-CD31 antibody and magnetic micro-beads, coupled to a derivative of biotin and streptavidin, respectively. Following magnetic bead separation, CD31-positive ECs were released by biotin-streptavidin competition. In the absence of micro-beads, EC

SUBMITTER: Sievert W 

PROVIDER: S-EPMC3954738 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature