Role of Shp2 in forebrain neurons in regulating metabolic and cardiovascular functions and responses to leptin.

ABSTRACT:

Objective

We examined whether deficiency of Src homology 2 containing phosphatase (Shp2) signaling in forebrain neurons alters metabolic and cardiovascular regulation under various conditions and if it attenuates the anorexic and cardiovascular effects of leptin. We also tested whether forebrain Shp2 deficiency alters blood pressure (BP) and heart rate (HR) responses to acute stress.

Design

Forebrain Shp2(-/-) mice were generated by crossing Shp2(flox/flox) mice with CamKIIα-cre mice. At 22-24 weeks of age, the mice were instrumented for telemetry for measurement of BP, HR and body temperature (BT). Oxygen consumption (VO2), energy expenditure and motor activity were monitored by indirect calorimetry.

Results

Shp2/CamKIIα-cre mice were heavier (46±3 vs 32±1 g), hyperg

SUBMITTER: do Carmo JM 

PROVIDER: S-EPMC3954949 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature