A multi-center ring trial of allergen analysis using fluorescent multiplex array technology.

ABSTRACT:

Background

Consistent performance of allergen assays is essential to ensure reproducibility of exposure assessments for investigations of asthma and occupational allergic disease. This study evaluated intra- and inter-laboratory reproducibility of a fluorescent multiplex array, which simultaneously measures eight indoor allergens in a single reaction well.

Methods

A multi-center study was performed in nine laboratories in the US and Europe to determine the inter-laboratory variability of an 8-plex array for dust mite, cat, dog, rat, mouse and cockroach allergens. Aliquots of 151 dust extract samples were sent to participating centers and analyzed by each laboratory on three separate occasions. Agreement within and between laboratories was calculated by the concordance correl

SUBMITTER: King EM 

PROVIDER: S-EPMC3955085 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature