Immobilization of actively thromboresistant assemblies on sterile blood-contacting surfaces.

ABSTRACT: Rapid one-step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point-of-care scheme is demonstrated herein to site-specifically immobilize azido-thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin-coated grafts in a primate model of acute graft thrombosis.

SUBMITTER: Qu Z 

PROVIDER: S-EPMC3955951 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature