Photoimprint photoacoustic microscopy for three-dimensional label-free subdiffraction imaging.

ABSTRACT: Subdiffraction optical microscopy allows the imaging of cellular and subcellular structures with a resolution finer than the diffraction limit. Here, combining the absorption-based photoacoustic effect and intensity-dependent photobleaching effect, we demonstrate a simple method for subdiffraction photoacoustic imaging of both fluorescent and nonfluorescent samples. Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited excitation volume, thus biasing the signal contributions from a second excitation pulse striking the same region. The differential signal between the two excitations preserves the signal contribution mostly from the center of the excitation volume, and dramatically

SUBMITTER: Yao J 

PROVIDER: S-EPMC3957272 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature