Identification of androgen-coregulated protein networks from the microsomes of human prostate cancer cells.

ABSTRACT:

Background

Androgens play a critical role in the development of prostate cancer-dysregulation of androgen-regulated growth pathways can led to hormone-refractory prostate cancer. A comprehensive understanding of androgen-regulated cellular processes has not been achieved to date. To this end, we have applied a large-scale proteomic approach to define cellular processes that are responsive to androgen treatment in LNCaP prostate cancer cells.

Results

Using isotope-coded affinity tags and mass spectrometry we identified and quantified the relative abundance levels of 1,064 proteins and found that distinct cellular processes were coregulated by androgen while others were essentially unaffected. Subsequent pharmacological perturbation of the cellular process for energy generatio

SUBMITTER: Wright ME 

PROVIDER: S-EPMC395736 | biostudies-literature | 2003

REPOSITORIES: biostudies-literature