CLoNe is a new method to target single progenitors and study their progeny in mouse and chick.

ABSTRACT: Cell lineage analysis enables us to address pivotal questions relating to: the embryonic origin of cells and sibling cell relationships in the adult body; the contribution of progenitors activated after trauma or disease; and the comparison across species in evolutionary biology. To address such fundamental questions, several techniques for clonal labelling have been developed, each with its shortcomings. Here, we report a novel method, CLoNe that is designed to work in all vertebrate species and tissues. CLoNe uses a cocktail of labelling, targeting and transposition vectors that enables targeting of specific subpopulations of progenitor types with a combination of fluorophores resulting in multifluorescence that describes multiple clones per specimen. Furthermore, transposition into the

SUBMITTER: Garcia-Moreno F 

PROVIDER: S-EPMC3957378 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature