High-throughput chemical screening for antivirulence developmental phenotypes in Trypanosoma brucei.

ABSTRACT: In the bloodstream of mammalian hosts, the sleeping sickness parasite, Trypanosoma brucei, exists as a proliferative slender form or a nonproliferative, transmissible, stumpy form. The transition between these developmental forms is controlled by a density-dependent mechanism that is important for the parasite's infection dynamics, immune evasion via ordered antigenic variation, and disease transmissibility. However, stumpy formation has been lost in most laboratory-adapted trypanosome lines, generating monomorphic parasites that proliferate uncontrolled as slender forms in vitro and in vivo. Nonetheless, these forms are readily amenable to cell culture and high-throughput screening for trypanocidal lead compounds. Here, we have developed and exploited a high-throughput screen for developm

SUBMITTER: MacGregor P 

PROVIDER: S-EPMC3957582 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature