A visual screen of protein localization during sporulation identifies new components of prospore membrane-associated complexes in budding yeast.

ABSTRACT: During ascospore formation in Saccharomyces cerevisiae, the secretory pathway is reorganized to create new intracellular compartments, termed prospore membranes. Prospore membranes engulf the nuclei produced by the meiotic divisions, giving rise to individual spores. The shape and growth of prospore membranes are constrained by cytoskeletal structures, such as septin proteins, that associate with the membranes. Green fluorescent protein (GFP) fusions to various proteins that associate with septins at the bud neck during vegetative growth as well as to proteins encoded by genes that are transcriptionally induced during sporulation were examined for their cellular localization during prospore membrane growth. We report localizations for over 100 different GFP fusions, including over 30 prote

SUBMITTER: Lam C 

PROVIDER: S-EPMC3957589 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature