High-yield, zero-leakage expression system with a translational switch using site-specific unnatural amino Acid incorporation.

ABSTRACT: Synthetic biologists construct complex biological circuits by combinations of various genetic parts. Many genetic parts that are orthogonal to one another and are independent of existing cellular processes would be ideal for use in synthetic biology. However, our toolbox is still limited with respect to the bacterium Escherichia coli, which is important for both research and industrial use. The site-specific incorporation of unnatural amino acids is a technique that incorporates unnatural amino acids into proteins using a modified exogenous aminoacyl-tRNA synthetase/tRNA pair that is orthogonal to any native pairs in a host and is independent from other cellular functions. Focusing on the orthogonality and independency that are suitable for the genetic parts, we designed novel AND gate and

SUBMITTER: Minaba M 

PROVIDER: S-EPMC3957627 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature