Incorporation of a proteotyping approach using mass spectrometry for surveillance of influenza virus in cell-cultured strains.

ABSTRACT: The reemergence of deadly pandemic influenza virus strains has necessitated the development of improved methods for rapid detection and subtyping of influenza viruses that will enable more strains to be characterized at the molecular level. Representative circulating strains of human influenza viruses from primary clinical specimens were grown in cell culture, purified through polyethylene glycol precipitation, proteolytically digested with an endoproteinase, and analyzed and identified by high-resolution mass spectrometry using unique signature peptides that are characteristic of type A H1N1 and H3N2 and type B influenza viruses. This proteotyping approach enabled circulating strains of type A influenza virus to be typed and subtyped, cocirculating seasonal and pandemic H1N1 viruses to be

SUBMITTER: Fernandes ND 

PROVIDER: S-EPMC3957765 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature