A new spreadsheet method for the analysis of bivariate flow cytometric data.

ABSTRACT:

Background

A useful application of flow cytometry is the investigation of cell receptor-ligand interactions. However such analyses are often compromised due to problems interpreting changes in ligand binding where the receptor expression is not constant. Commonly, problems are encountered due to cell treatments resulting in altered receptor expression levels, or when cell lines expressing a transfected receptor with variable expression are being compared. To overcome this limitation we have developed a Microsoft Excel spreadsheet that aims to automatically and effectively simplify flow cytometric data and perform statistical tests in order to provide a clearer graphical representation of results.

Results

To demonstrate the use and advantages of this new spreadsheet method we

SUBMITTER: Tzircotis G 

PROVIDER: S-EPMC395826 | biostudies-literature | 2004 Mar

REPOSITORIES: biostudies-literature