Preparation of colloidal gold immunochromatographic strip for detection of Paragonimiasis skrjabini.

ABSTRACT:

Background

Paragonimiasis is a food-borne trematodiasis, a serious public health issue and a neglected tropical disease. Paragonimus skrjabini is a unique species found in China. Unlike paragonimiasis westermani, it is nearly impossible to make a definitive diagnosis for paragonimiasis skrjabini by finding eggs in sputum or feces. Immunodiagnosis is the best choice to detect paragonimiasis skrjabini. There is an urgent need to develop a novel, rapid and simple immunoassay for large-scale screening patients in endemic areas.

Methodology/principal findings

To develop a rapid, simple immunodiagnostic assay for paragonimiasis, rabbit anti-human IgG was conjugated to colloidal gold particles and used to detect antibodies in the sera of paragonimiasis patients. The synthesis and i

SUBMITTER: Wang Y 

PROVIDER: S-EPMC3958401 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature