Low levels of antibody-dependent enhancement in vitro using viruses and plasma from dengue patients.

ABSTRACT:

Background

The majority of dengue patients infected with any serotype of dengue virus (DENV) are asymptomatic, but the remainder may develop a wide spectrum of clinical symptoms, ranging from mild dengue fever (DF) to severe dengue hemorrhagic fever (DHF). Severe cases occur more often in patients who experience a secondary infection with a different virus serotype. A phenomenon called antibody-dependent enhancement (ADE) has been proposed to explain the onset of these severe cases, but the exact mechanism of ADE remains unclear.

Methodology/principal finding

Virus neutralization and ADE assays were performed using ultracentrifugation supernatants of acute-phase sera from patients with secondary infections or human monoclonal antibodies (HuMAbs) as anti-DENV antibodies. Viru

SUBMITTER: Chaichana P 

PROVIDER: S-EPMC3958444 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature