Gold nanoparticle-M2e conjugate coformulated with CpG induces protective immunity against influenza A virus.

ABSTRACT:

Aim

This study aimed to develop a novel influenza A vaccine by conjugating the highly conserved extracellular region of the matrix 2 protein (M2e) of influenza A virus to gold nanoparticles (AuNPs) and to test the vaccine in a mouse influenza challenge model.

Materials & methods

Citrate-reduced AuNPs (diameter: 12 nm) were synthesized, and characterized by transmission electron microscopy and dynamic light scattering. M2e was conjugated to AuNPs through thiol-gold interactions to form M2e-AuNP conjugates. Particle stability was confirmed by UV-visible spectra, and M2e conjugation was further characterized by x-ray photoelectron spectroscopy. Mice were immunized with M2e-AuNPs with or without CpG (cytosine-guanine rich oligonucleotide) as an adjuvant with appropriate control

SUBMITTER: Tao W 

PROVIDER: S-EPMC3958969 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature