Rapid strategy for screening by pyrosequencing of influenza virus reassortants--candidates for live attenuated vaccines.

ABSTRACT:

Background

Live attenuated influenza vaccine viruses (LAIVs) can be generated by classical reassortment of gene segments between a cold adapted, temperature sensitive and attenuated Master Donor Virus (MDV) and a seasonal wild-type (wt) virus. The vaccine candidates contain hemagglutinin (HA) and neuraminidase (NA) genes derived from the circulating wt viruses and the remaining six genes derived from the MDV strains. Rapid, efficient selection of the viruses with 6∶2 genome compositions from the large number of genetically different viruses generated during reassortment is essential for the biannual production schedule of vaccine viruses.

Methodology/principal findings

This manuscript describes a new approach for the genotypic analysis of LAIV reassortant virus clones based

SUBMITTER: Shcherbik SV 

PROVIDER: S-EPMC3960276 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature