Selective microtubule-based transport of dendritic membrane proteins arises in concert with axon specification.

ABSTRACT: The polarized distribution of membrane proteins to axonal or somatodendritic neuronal compartments is fundamental to nearly every aspect of neuronal function. The polarity of dendritic proteins depends on selective microtubule-based transport; the vesicles that carry these proteins are transported into dendrites but do not enter the axon. We used live-cell imaging of fluorescently tagged dendritic and axonal proteins combined with immunostaining for initial segment and cytoskeletal markers to evaluate different models of dendrite-selective transport in cultured rat hippocampal neurons. In mature neurons, dendritic vesicles that entered the base of the axon stopped at the proximal edge of the axon initial segment, defined by immunostaining for ankyrinG, rather than moving into the initial s

SUBMITTER: Petersen JD 

PROVIDER: S-EPMC3960460 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature