Quantitative proteomics in resected renal cancer tissue for biomarker discovery and profiling.

ABSTRACT:

Background

Proteomics-based approaches for biomarker discovery are promising strategies used in cancer research. We present state-of-art label-free quantitative proteomics method to assess proteome of renal cell carcinoma (RCC) compared with noncancer renal tissues.

Methods

Fresh frozen tissue samples from eight primary RCC lesions and autologous adjacent normal renal tissues were obtained from surgically resected tumour-bearing kidneys. Proteins were extracted by complete solubilisation of tissues using filter-aided sample preparation (FASP) method. Trypsin digested proteins were analysed using quantitative label-free proteomics approach followed by data interpretation and pathways analysis.

Results

A total of 1761 proteins were identified and quantified with high co

SUBMITTER: Atrih A 

PROVIDER: S-EPMC3960606 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature