Long non-coding RNA and alternative splicing modulations in Parkinson's leukocytes identified by RNA sequencing.

ABSTRACT: The continuously prolonged human lifespan is accompanied by increase in neurodegenerative diseases incidence, calling for the development of inexpensive blood-based diagnostics. Analyzing blood cell transcripts by RNA-Seq is a robust means to identify novel biomarkers that rapidly becomes a commonplace. However, there is lack of tools to discover novel exons, junctions and splicing events and to precisely and sensitively assess differential splicing through RNA-Seq data analysis and across RNA-Seq platforms. Here, we present a new and comprehensive computational workflow for whole-transcriptome RNA-Seq analysis, using an updated version of the software AltAnalyze, to identify both known and novel high-confidence alternative splicing events, and to integrate them with both protein-domains a

SUBMITTER: Soreq L 

PROVIDER: S-EPMC3961179 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature