ATM alters the otherwise robust chromatin mobility at sites of DNA double-strand breaks (DSBs) in human cells.

ABSTRACT: Ionizing radiation induces DNA double strand breaks (DSBs) which can lead to the formation of chromosome rearrangements through error prone repair. In mammalian cells the positional stability of chromatin contributes to the maintenance of genome integrity. DSBs exhibit only a small, submicron scale diffusive mobility, but a slight increase in the mobility of chromatin domains by the induction of DSBs might influence repair fidelity and the formation of translocations. The radiation-induced local DNA decondensation in the vicinity of DSBs is one factor potentially enhancing the mobility of DSB-containing chromatin domains. Therefore in this study we focus on the influence of different chromatin modifying proteins, known to be activated by the DNA damage response, on the mobility of DSBs. IR

SUBMITTER: Becker A 

PROVIDER: S-EPMC3961414 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature