In multiple sclerosis, oligoclonal bands connect to peripheral B-cell responses.

ABSTRACT:

Objective

To determine to what extent oligoclonal band (OCB) specificities are clonally interrelated and to what degree they are associated with corresponding B-cell responses in the peripheral blood (PB) of multiple sclerosis (MS) patients.

Methods

Mass-spectrometric proteomic analysis of isoelectric focused (IEF) cerebrospinal fluid (CSF) immunoglobulin G (IgG) was used in combination with next-generation deep-immune repertoire sequencing of PB and CSF IgG heavy chain variable regions from MS patients.

Results

We find evidence for ongoing stimulation and maturation to antibody-expressing B cells to occur primarily inside the central nervous system (CNS) compartment. B cells participating in OCB production can also be identified in PB; these cells appear to migrate a

SUBMITTER: Bankoti J 

PROVIDER: S-EPMC3961546 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature