Next generation MUT-MAP, a high-sensitivity high-throughput microfluidics chip-based mutation analysis panel.

ABSTRACT: Molecular profiling of tumor tissue to detect alterations, such as oncogenic mutations, plays a vital role in determining treatment options in oncology. Hence, there is an increasing need for a robust and high-throughput technology to detect oncogenic hotspot mutations. Although commercial assays are available to detect genetic alterations in single genes, only a limited amount of tissue is often available from patients, requiring multiplexing to allow for simultaneous detection of mutations in many genes using low DNA input. Even though next-generation sequencing (NGS) platforms provide powerful tools for this purpose, they face challenges such as high cost, large DNA input requirement, complex data analysis, and long turnaround times, limiting their use in clinical settings. We report th

SUBMITTER: Schleifman EB 

PROVIDER: S-EPMC3962342 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature