Characterisation and comparison of lactating mouse and bovine mammary gland miRNomes.

ABSTRACT:

Background

The mammary gland is a dynamic organ that undergoes important physiological changes during reproductive cycles. Until now, data regarding the characterisation of miRNA in the mammary gland have been scarce and mainly focused on their abnormal expression in breast cancer. Our goal was to characterise the microRNA (miRNA) involved in mechanisms regulating the mammary function, with particular focus on the lactation stage.

Methodology/principal findings

Using high-throughput sequencing technology, the exhaustive repertoires of miRNA expressed (miRNome) in mouse and bovine mammary glands during established lactation were identified, characterized and compared. Furthermore, in order to obtain more information on miRNA loading in the RNA-induced silencing complex (RISC)

SUBMITTER: Le Guillou S 

PROVIDER: S-EPMC3962357 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature