Protection against dengue virus infection in mice by administration of antibodies against modified nonstructural protein 1.

ABSTRACT:

Background

Infection with dengue virus (DENV) may cause life-threatening disease with thrombocytopenia and vascular leakage which are related to dysfunction of platelets and endothelial cells. We previously showed that antibodies (Abs) against DENV nonstructural protein 1 (NS1) cross-react with human platelets and endothelial cells, leading to functional disturbances. Based on sequence homology analysis, the C-terminal region of DENV NS1 protein contains cross-reactive epitopes. For safety in vaccine development, the cross-reactive epitopes of DENV NS1 protein should be deleted or modified.

Methodology/principal findings

We tested the protective effects of Abs against full-length DENV NS1, NS1 lacking the C-terminal amino acids (a.a.) 271-352 (designated ΔC NS1), and chimeri

SUBMITTER: Wan SW 

PROVIDER: S-EPMC3962419 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature