High concentrations of methyl fluoride affect the bacterial community in a thermophilic methanogenic sludge.

ABSTRACT: To precisely control the application of methyl fluoride (CH3F) for analysis of methanogenic pathways, the influence of 0-10% CH3F on bacterial and archaeal communities in a thermophilic methanogenic sludge was investigated. The results suggested that CH3F acts specifically on acetoclastic methanogenesis. The inhibitory effect stabilized at an initial concentration of 3-5%, with around 90% of the total methanogenic activity being suppressed, and a characteristic of hydrogenotrophic pathway in isotope fractionation was demonstrated under this condition. However, extended exposure (12 days) to high concentrations of CH3F (>3%) altered the bacterial community structure significantly, resulting in increased diversity and decreased evenness, which can be related to acetate oxidation and CH3F deg

SUBMITTER: Hao L 

PROVIDER: S-EPMC3962445 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature