The dynamics of diverse segmental amplifications in populations of Saccharomyces cerevisiae adapting to strong selection.

ABSTRACT: Population adaptation to strong selection can occur through the sequential or parallel accumulation of competing beneficial mutations. The dynamics, diversity, and rate of fixation of beneficial mutations within and between populations are still poorly understood. To study how the mutational landscape varies across populations during adaptation, we performed experimental evolution on seven parallel populations of Saccharomyces cerevisiae continuously cultured in limiting sulfate medium. By combining quantitative polymerase chain reaction, array comparative genomic hybridization, restriction digestion and contour-clamped homogeneous electric field gel electrophoresis, and whole-genome sequencing, we followed the trajectory of evolution to determine the identity and fate of beneficial mutati

SUBMITTER: Payen C 

PROVIDER: S-EPMC3962480 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature