A systematic assessment of normalization approaches for the Infinium 450K methylation platform.

ABSTRACT: The Illumina Infinium HumanMethylation450 BeadChip has emerged as one of the most popular platforms for genome wide profiling of DNA methylation. While the technology is wide-spread, systematic technical biases are believed to be present in the data. For example, this array incorporates two different chemical assays, i.e., Type I and Type II probes, which exhibit different technical characteristics and potentially complicate the computational and statistical analysis. Several normalization methods have been introduced recently to adjust for possible biases. However, there is considerable debate within the field on which normalization procedure should be used and indeed whether normalization is even necessary. Yet despite the importance of the question, there has been little comprehensive c

SUBMITTER: Wu MC 

PROVIDER: S-EPMC3962542 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature