De novo synthesis of milk triglycerides in humans.

ABSTRACT:

Unlabelled

Mammary gland (MG) de novo lipogenesis contributes significantly to milk fat in animals but little is known in humans.

Objective

To test the hypothesis that the incorporation of (13)C carbons from [U-(13)C]glucose into fatty acids (FA) and glycerol in triglycerides (TG) will be greater: 1) in milk than plasma TG, 2) during a high-carbohydrate (H-CHO) diet than high-fat (H-FAT) diet, and 3) during feeding than fasting. Seven healthy, lactating women were studied on two isocaloric, isonitrogenous diets. On one occasion, subjects received diets containing H-FAT or H-CHO diet for 1 wk. Incorporation of (13)C from infused [U-(13)C]glucose into FA and glycerol was measured using GC-MS and gene expression in RNA isolated from milk fat globule using microarrays. Incorpora

SUBMITTER: Mohammad MA 

PROVIDER: S-EPMC3962611 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature