Expression and function of NIK- and IKK2-binding protein (NIBP) in mouse enteric nervous system.

ABSTRACT:

Background

NIK- and IKK2-binding protein (NIBP)/TRAPPC9 is expressed in brain neurons, and human NIBP mutations are associated with neurodevelopmental disorders. The cellular distribution and function of NIBP in the enteric nervous system (ENS) remain unknown.

Methods

Western blot and reverse transcription-polymerase chain reaction analysis were used respectively to identify the protein and mRNA expression of NIBP and other neuronal markers. Multi-labeled immunofluorescent microscopy and confocal image analysis were used to examine the cellular distribution of NIBP-like immunoreactivity (IR) in whole mount intestine. Enteric neuronal cell line (ENC) was infected with lentivirus carrying NIBP or its shRNA expression vectors and treated with vehicle or tumor necrosis factor (T

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC3962790 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature