Coefficient of Variation, Signal-to-Noise Ratio, and Effects of Normalization in Validation of Biomarkers from NMR-based Metabonomics Studies.

ABSTRACT: A primary goal of metabonomics research is biomarker discovery for human diseases based on differences in metabolic profiles between healthy and diseased patient populations. One of the most significant challenges in biomarker discovery is validation, which implicitly depends on the coefficient of variation (CV) associated with the measurement technique. This paper investigates how the CV of metabolite resonances measured by nuclear magnetic resonance spectroscopy (NMR) depends on signal-to-noise ratio (SNR) and normalization method. CVs were calculated for NMR resonance peaks in a series of NMR spectra of five synthetic urine samples collected over an eight-month period. An inverse correlation was detected between SNR and CV for all normalization methods. Small peaks with SN

SUBMITTER: Wang B 

PROVIDER: S-EPMC3963315 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature