Exploration of genome-wide circulating microRNA in hepatocellular carcinoma: MiR-483-5p as a potential biomarker.

ABSTRACT:

Background

MicroRNAs (miRNA) are abundant in the circulation and play a central role in diverse biologic processes; they may be useful for early diagnosis of hepatocellular carcinoma.

Methods

We conducted a two-phase, case-control study (20 pairs for the discovery set and 49 pairs for the validation set) to test the hypothesis that genome-wide dysregulation of circulating miRNAs differentiates hepatocellular carcinoma cases from controls. Taqman low-density arrays were used to examine genome-wide miRNA expression for the discovery set, and quantitative real-time PCR was used to validate candidate miRNAs for both discovery and validation sets.

Results

Sixty-six miRNAs were found to be significantly overexpressed in plasma of hepatocellular carcinoma cases compared with

SUBMITTER: Shen J 

PROVIDER: S-EPMC3963823 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature