Single-molecule studies of polymerase dynamics and stoichiometry at the bacteriophage T7 replication machinery.

ABSTRACT: Replication of DNA plays a central role in transmitting hereditary information from cell to cell. To achieve reliable DNA replication, multiple proteins form a stable complex, known as the replisome, enabling them to act together in a highly coordinated fashion. Over the past decade, the roles of the various proteins within the replisome have been determined. Although many of their interactions have been characterized, it remains poorly understood how replication proteins enter and leave the replisome. In this study, we visualize fluorescently labeled bacteriophage T7 DNA polymerases within the replisome while we simultaneously observe the kinetics of the replication process. This combination of observables allows us to monitor both the activity and dynamics of individual polymerases durin

SUBMITTER: Geertsema HJ 

PROVIDER: S-EPMC3964090 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature