In Vivo and in vitro dynamics of undifferentiated embryonic cell transcription factor 1.

ABSTRACT: Pluripotent stem cells retain the ability to differentiate into the three germ layers and germline. As a result, there is a major interest in characterizing regulators that establish and maintain pluripotency. The network of transcription factors continues to expand in complexity, and one factor, undifferentiated embryonic cell transcription factor 1 (UTF1), has recently moved more into the limelight. To facilitate the study of UTF1, we report the generation and characterization of two reporter lines that enable efficient tracking, mapping, and purification of endogenous UTF1. In particular, we include a built-in biotinylation system in our targeted locus that allows efficient and reliable pulldown. We also use this reporter to show the dynamic regulation of Utf1 in distinct stem cell cond

SUBMITTER: Galonska C 

PROVIDER: S-EPMC3964277 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature