Platform for induction and maintenance of transgene-free hiPSCs resembling ground state pluripotent stem cells.

ABSTRACT: Cell banking, disease modeling, and cell therapy applications have placed increasing demands on hiPSC technology. Specifically, the high-throughput derivation of footprint-free hiPSCs and their expansion in systems that allow scaled production remains technically challenging. Here, we describe a platform for the rapid, parallel generation, selection, and expansion of hiPSCs using small molecule pathway inhibitors in stage-specific media compositions. The platform supported efficient and expedited episomal reprogramming using just OCT4/SOX2/SV40LT combination (0.5%-4.0%, between days 12 and 16) in a completely feeder-free environment. The resulting hiPSCs are transgene-free, readily cultured, and expanded as single cells while maintaining a homogeneous and genomically stable pluripotent pop

SUBMITTER: Valamehr B 

PROVIDER: S-EPMC3964282 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature