Murine cytomegalovirus infection and apoptosis in organotypic retinal cultures.

ABSTRACT:

Purpose

An organotypic retinal culture model was used to determine the pattern of murine cytomegalovirus (MCMV) infection and whether apoptosis is induced in MCMV-infected cultured retinas.

Methods

Retinas harvested from C57BL/6 mice were individually cultured at 37 degrees C on 3-microm filter inserts placed in 24-well plates. Some retinas were infected with MCMV (5 x 10(5) PFU/well). At days 4, 7, and 11 after infection (pi), the culture medium and cultured retinas were collected for examination.

Results

Replicating virus was recovered and viral early antigen (EA)- and late antigen (LA)-positive cells were observed in the MCMV-infected retinal cultures. Most MCMV-infected cells were glia and horizontal cells. Infection resulted in atrophy of the photoreceptor cells

SUBMITTER: Zhang M 

PROVIDER: S-EPMC3964603 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature