Peptidergic regulation of plasminogen activator inhibitor-1 gene expression in vivo.

ABSTRACT:

Background

The mechanisms by which PAI-1 biosynthesis is altered during stress have not been fully elucidated. Studies suggest a major role for neuro-peptidergic modulation of the stress response by PACAP (pituitary adenylate cyclase-activating polypeptide), a member of the VIP/secretin/glucagon family.

Objective

We tested the hypothesis that PACAP regulates PAI-1 biosynthesis during stress in vivo.

Methods

PAI-1 gene expression was monitored by RT-PCR in adrenal glands harvested from C57BL/6J mice that were unstressed, or subjected to restraint stress for 2 h, or treated with PACAP.

Results

PAI-1 mRNA expression was markedly increased in adrenals from stressed mice. Restraint stress resulted in much smaller increments in adrenal tPA mRNA, suggesting that local

SUBMITTER: Gingles NA 

PROVIDER: S-EPMC3965269 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature