Combining decellularized human adipose tissue extracellular matrix and adipose-derived stem cells for adipose tissue engineering.

ABSTRACT: Repair of soft tissue defects resulting from lumpectomy or mastectomy has become an important rehabilitation process for breast cancer patients. This study aimed to provide an adipose tissue engineering platform for soft tissue defect repair by combining decellularized human adipose tissue extracellular matrix (hDAM) and human adipose-derived stem cells (hASCs). To derive hDAM incised human adipose tissues underwent a decellularization process. Effective cell removal and lipid removal were proved by immunohistochemical analysis and DNA quantification. Scanning electron microscopic examination showed a three-dimensional nanofibrous architecture in hDAM. The hDAM included collagen, sulfated glycosaminoglycan, and vascular endothelial growth factor, but lacked major histocompatibility complex

SUBMITTER: Wang L 

PROVIDER: S-EPMC3965366 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature